On the first phase, Vitreous will have its smaller eyes attack Link. Each collision will deal four hearts of damage. The smaller eyes have 48 HP per eye. On occasion, the big eye will shoot lightning, which deals two hearts of damage. The arrows deal Golden Sword damage to it.
On the second phase, the big eye of Vitreous emerges. It will chase Link around the room.
It is recommended to do a spin attack on the first phase with the Tempered Sword. Stand near the acid by the left eyes, but not directly on it to avoid damage. Use arrows if necessary. On the second phase, use arrows to push Vitreous back so it doesn't damage Link.